Notes on HYPOMYCES in the Pacific Northwest

Prepared for the Pacific Northwest Key Council
By Ian Gibson, South Vancouver Island Mycological Society
Copyright © 2008, 2017 Pacific Northwest Key Council
Photo copyright held by each photographer
Do not copy photos without permission

TABLE OF CONTENTS

 Introduction

 Key to Species

 Descriptions

 Glossary

 References

 Index

INTRODUCTION

The purpose of this key is to present information that will be useful to those seeking to identify Hypomyces species in the field. Specialists use microscopic criteria, and these should be the final arbiter, but it is often possible to come to a reasonable conclusion without resort to the microscope, based on color and a recognizable host.

The most familiar Hypomyces in the Pacific Northwest is Hypomyces lactifluorum, the edible “lobster mushroom”. Named in English for its orange-red color rather than its taste, it fruits as a layer on Russula brevipes mushrooms. As it does so, it interferes with the development of the Russula, so that the gills are not well formed. Hypomyces species form on the surface of mushrooms, partially or completely covering them with a layer of powdery to pimpled tissue, disfiguring the mushroom as they do so.

Many mushrooms are affected by Hypomyces species: Rogerson & Samuels wrote a series of 3 articles describing 10 world species that grow on boletes, 19 species that grow on polypores, and 13 that grow on gilled mushrooms. The most familiar of the other species in the Pacific Northwest are H. chrysospermus, the “bolete eater”, that forms a white to yellow layer on boletes, H. aurantius that forms an orange layer on old polypores, and H. cervinigenus that forms a white to pink layer on Helvella lacunosa.

Hypomyces hyalinus, the “amanita eater” selects Amanita mushrooms as its preferred host. Hypomyces luteovirens is yellow or green as suggested by its Latin name. Hypomyces rosellus is white to “rose”, a bright pinkish red. Hypomyces lateritius has a variety of colors including the brick red suggested by its name. Hypomyces ochraceus is rarely seen in its colored phase.

The sexual form of these fungi (teleomorph) bears ascospores in asci which develop in perithecia. A perithecium is a nearly spherical, ovoid, pear-shaped or beaked (flask-like) body bearing asci in the interior with or without sterile paraphyses, with an opening to the air called an ostiole. The ostiole is generally visible under a hand lens as a small pit or pimple on the surface of the Hypomyces. Other fungi in the Pacific Northwest that have perithecia include Xylaria and Cordyceps.

Most species have an asexual form classified with the mitosporic fungi (anamorphic fungi). In the case of Hypomyces chrysospermus, H. cervinigenus, and H. ochraceus, this form is much more likely to be encountered than the sexual form.

KEY TO SPECIES

1a Bright red-orange

................................................................................2

1b Different in color (white, yellow, yellowish orange, brick red, reddish black, ochraceous, brown, but not bright orange-red)

................................................................................3

2a Growing on decayed mushrooms (Russula or Lactarius) on the ground

................................................................................Hypomyces lactifluorum

2b Growing on polypores or other mushrooms that grow on wood

................................................................................Hypomyces aurantius

3a (1b) Pinkish brown, growing on Helvella

................................................................................Hypomyces cervinigenus

3b Color different or host different or host unrecognizable

................................................................................4

4a Green or yellow green (growing on Russula or Lactarius)

................................................................................Hypomyces luteovirens

4b Color different or host different or host unrecognizable

................................................................................5

5a White to yellow or rarely red-brown, and growing on recognizable bolete

................................................................................Hypomyces chrysospermus

5b Color different or host different or host unrecognizable

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6a White, pallid when old or tinged with pink, yellow, or brown, growing on recognizable Amanita

................................................................................Hypomyces hyalinus

6b Color different or host different or host unrecognizable

................................................................................7

7a Color white becoming rose-colored or red

................................................................................Hypomyces rosellus

7b Color not becoming rose-colored or red (may be brick red or reddish black)

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8a Color white to lemon yellow when fresh, buff, yellowish orange, tawny, at full maturity becoming ochraceous, brick red and brown or reddish black, growing on gills of Lactarius species

................................................................................Hypomyces lateritius

8b Color different or host different or host unrecognizable

................................................................................9

9a White, growing on Russula or Lactarius, no pits or pimples under hand lens

................................................................................Hypomyces ochraceus, Hypomyces lateritius

9b Color white or different or host different or host unrecognizable

................................................................................microscopic identification needed

DESCRIPTIONS

Hypomyces aurantius

FEATURES orange layer of perithecia forming small pimples on surface, growing over polypores and other fungi, turning violet to purplish red in KOH. DETAILS It is usually seen as floccose layer, often covering entire host, occasionally spreading over adjacent substrate, “typically yellowish-orange, orange, red to rusty-red, varying from almost white to buff, through many shades of orange and red", turning violet to purplish red in KOH, (Rogerson & Samuels). Anamorph (Cladobotrum varium Nees) is a profuse pure white cottony-powdery mat. FRUITING on many species of fungi, especially polypores and others growing on wood, fairly common. MICROSTRUCTURES ascospores (13)20-25(27) x (3)4-6(7.5) um, fusiform to lanceolate, verrucose (verrucae <= 0.5 um high), 2-celled, apiculi 2-4.5 um long, acute; perithecium turning purplish in 3% KOH.

Hypomyces aurantius © Zoey Green

Hypomyces cervinigenus

FEATURES The anamorph, the usual form seen, produces a white covering on Helvella species, parasitizing cap and stem tissues, the covering becoming pinkish brown to cocoa brown. DETAILS The anamorph forms a soft, cottony to finely downy, mould-like covering, white becoming pinkish to cream-buff, powdery when old, not changing color in KOH. FRUITING on Helvella species, especially Helvella lacunosa, fairly common. MICROSTRUCTURES Two types of asexual spores are formed: 1) aleuriospores 13.5-17.5 um in diameter including ornamentation, round, spiny, thick-walled, joined to secondary, thin-walled, more or less hemispheric cell, (in deposit the aleuriospores are pinkish buff), 2) conidia 14.0-25.5 x 4.0-5.0 um, cylindric-elliptic, smooth, thin-walled, contents granular or with one to several oil droplets, some centrally septate. Ascospores and perithecia are rare, the ascospores (15)18-22(26) x 2-4(5) um, subfusiform, smooth, at maturity typically 1-septate, the ends pointed, not apiculate.

Hypomyces cervinigenus © Fred Stevens (MykoWeb)

Hypomyces chrysospermus     bolete-eater, golden Hypomyces

FEATURES A three stage mould-like growth on fungi of Boletaceae, the first white, the second yellow, and the third (seldom seen) red-brown and pimpled; covering the cap, stem and tube surfaces of the host and filling the tubes, the host fruitbody becoming decayed (soft and mushy). DETAILS The anamorph is known as Verticillium in the white stage and as Sepedonium chrysospermum in the yellow stage. Formation begins as a white mouldy-looking covering "that attacks and quickly engulfs boletes, then turns bright yellow and powdery", and then finally becomes reddish brown and pimpled (but this final stage rarely seen, only occurring after host is decayed beyond recognition and unpleasant to handle), (Arora). Subiculum is "at first white, usually becoming golden yellow, remaining white or becoming pink, vinaceous to ferruginous"; perithecia "orange-yellow, brick red, red-brown, or rufous brown”, (Rogerson & Samuels). FRUITING grows on members of the Boletaceae, June to September, the anamorph stage common. MICROSTRUCTURES conidia especially in white stage, elliptic, smooth, (Rogerson & Samuels gives conidia as (4)7-13.3(20) x (2.5)3.3-5.5(7) um, Põldmaa et al. as 7-20(25) x 3-10 um, Arora 10-30 x 5-12 um), aleuriospores especially in yellow stage 10-25 um, round, thick-walled, and warted, ascospores in final stage spindle-shaped to lanceolate, verrucose, colorless, usually 1-septate, apiculi acute, longer at upper end than at lower end, (Rogerson & Samuels gives ascospore size as (15)20-25(30) x 4-5(6) um, Põldmaa et al. (13)15-20(25) x 4-6 um, Arora 25-30 x 5-6 um). REMARKS Hypomyces ochraceus is occasionally found on boletes. Hypomyces microspermus Rogerson & Samuels is "the most frequently collected of the boleticolous species in North America" (whereas H. chrysospermus is "the most frequently reported boleticolous species"): H. microspermus, found at least in California (Del Norte Co. in extreme northwest of state), eastern North America, and elsewhere in the world, has ascospores rarely above 15 um in length, not prominently verrucose, varying from equally bicellular to unequally bicellular, whereas those of H. chrysospermus rarely range below 20 um in length, are prominently verrucose, and unequally bicellular, conidia and aleuriospores of H. microspermus averaging somewhat smaller than those of H. chrysospermus but the measurements overlapping, the size of the conidia of H. microspermus given both as 11-14 x 4-5 um and as (5)6.4-11.7(14) x (2.5)2.7-4.5(5) um, size of aleuriospores given as 12-15 um in diameter, (Rogerson & Samuels).

Hypomyces chrysospermus © Steve Trudell

Hypomyces hyalinus     Amanita mold

FEATURES a mould-like covering with pimpled surface, formed on Amanita species, usually covering entire host, the host typically unexpanded with cap completely deformed and fused to stem, the gills obliterated, and the consistency of the fruitbody firm, the color white, pallid when old or tinged with pink, yellow, or brown, no anamorph definitely associated. FRUITING on Amanita species, June to October. MICROSTRUCTURES ascospores 15-20 x 4.5-6.5 um, fusiform, subcymbiform to oblanceolate, prominently verrucose (1-1.5 um high), 2-celled, septum near the base, apiculi acute to obtuse.

Hypomyces hyalinus © Eleanor Yarrow

Hypomyces lactifluorum    lobster mushroom

FEATURES bright orange to reddish color and smoothly pimpled surface, growth on distorted Russula brevipes (and other Russula and Lactarius spp.) whose gills are often reduced to blunt ridges, the growth usually covering the aborted cap and stem as well as gills, anamorph not found in nature. DETAILS "a layer of roughened or pimpled, bright orange to orange-red to purple-red or occasionally yellow-orange tissue which is firm to the touch", (Arora). FRUITING usually on the fruiting bodies of Lactarius and Russula (especially Russula brevipes), predominantly in fall, common, found throughout North America. MICROSTRUCTURES ascospores 30-50 x 4.5-8 um, spindle-shaped or shaped like caraway seeds, colorless, warted, 1-septate, (Arora), ascospores 35-40 x 4.5-7 um, fusiform, prominently verrucose and apiculate, 2-celled, perithecia KOH+ purple, (Rogerson & Samuels), ascospores 35-45 x 5-8 um, fusiform, with one side sometimes flattened, verrucose, verrucae low and confluent, 1-1.5 um high, ends apiculate, spores 1-septate, (Põldmaa et al.). REMARKS Hypomyces macrosporus Seaver (found at least in WA, Mexico, and eastern North America) is distinguishable only by color (subiculum white to buff, perithecia buff to yellowish amber) and negative reaction to KOH and may be merely an ‘albino’ form of this species: orange specimens of H. lactifluorum have been found mixed with specimens that were variously white, white with orange streaks and orange-red with white streaks, but only the reddish streaks of the whitish form became purple in KOH.

Hypomyces lactifluorum © Peter Katsaros

Hypomyces lateritius

FEATURES growth on gills of Lactarius species, covering the host gills and turning the host fruitbody firm, color white to lemon yellow when fresh, buff, yellowish orange, tawny, at full maturity becoming ochraceous, brick red and brown or reddish black, surface pimpled from perithecia; the whitish cottony anamorph Acremonium tulasnei G.R.W. Arnold may be found on host's mummified fruitbody before perithecia or in early stages. FRUITING on Lactarius species, found in British Columbia, Washington, Oregon, and Idaho, apparently worldwide where Lactarius species occur. MICROSTRUCTURES ascospores (11.2)15-21(30) x (2.6)3.6-4.8(6.0) um, (Rogerson & Samuels), (15)21-27(30) x (3)4-5.5(6) um (Põldmaa et al.), fusiform, lanceolate, or boat-shaped, with warts less than 0.5 um high, 1-celled but with inclusions giving the appearance of a median septum, with conspicuous acute apiculus at each end; perithecia KOH-; conidia (4.5)5-6.5(7.5) x 4.5-6(7) um, round or nearly round, equilateral, aseptate. REMARKS a variable taxon that may prove to be a complex of species.

Hypomyces lateritius © Michael Beug

Hypomyces luteovirens     yellow-green Hypomyces

FEATURES yellow or green growth on Russula spp. and occasionally Lactarius, covering deformed gills and often stem, sometimes cap, turning fruitbody firm, anamorph not known in nature. DETAILS subiculum at first whitish to yellowish to bright yellow, then yellowish green to dark green; perithecia yellow when fresh, drying olivaceous to blackish, usually darker than surrounding subiculum, immersed except for papilla. FRUITING on Russula species, also reported on Lactarius, July through November, common throughout many parts of North America. MICROSTRUCTURES ascospores 32-35 x 4.5-5.5 um, fusiform to naviculate, nearly smooth to prominently verrucose (verrucae up to 1 um high), one-celled, ends apiculate; perithecia KOH-.

Hypomyces luteovirens © Steve Trudell

Hypomyces ochraceus

FEATURES profuse, white, cottony, mould-like growth on Lactarius and Russula, or occasionally on boletes, covering spore-bearing surface or usually the whole fruitbody, the fruitbody becoming decayed, often extending over nearby substrate, rarely forming white, buff, or orange teleomorph with pimpled surface (usually on litter, mosses, ground, wood or bark after host destroyed). FRUITING the anamorph (Cladobotrum verticillatum (Link) S.J. Hughes) is common in eastern North America and recorded from Oregon, the teleomorph rare. MICROSTRUCTURES up to three types of "spores": a) conidia 11-24 x 7-12 um, of various shapes, b) chlamydospores (thick-walled cells) about 20 um in diameter with wall 2 um thick, and c) when rare teleomorph is present ascospores that are 32-40 x 5-8 um, 2-celled, verrucose, apiculate.

Hypomyces rosellus

FEATURES mould-like, cottony growth on many kinds of fungi, especially Aphyllophorales, at first white becoming pink, rose-colored or red, with dark red pimple-like perithecia. FRUITING on many kinds of fungi, spreading over a considerable area of the fungus host, often spreading out on the substrate of the host, covering host's spore-bearing surface or also host's cap and stem, host fruitbody becomes decayed or with no apparent change observed, recorded on Agaricus, Amanita, Armillaria, Clitocybe, Coprinus, Corticium, Crepidotus, Fomitopsis, Gomphidius, Gymnopus, Hydnellum, Hymenochaete, Hymenogaster, Hyphoderma, Inocybe, Inonotus, Lactarius, Nectria, Phellinus, Phlebiopsis, Piptoporus, Polyporus, Russula, Stereum, Trichaptum, Tricholomopsis; found at least British Columbia, California, both the teleomorph and anamorph common where it occurs. MICROSTRUCTURES ascospores (20)25-30(38) x (3)4-5(7.5) um, (Rogerson & Samuels), 25-35 x 4-6(7.5) um (Põldmaa et al.), fusiform to lanceolate, prominently verrucose (verrucae up to 1 um high), two-celled, ends conspicuously apiculate; anamorph in nature forming a delicate yellowish to red mycelium on which erect conidiophores are standing, each conidiophore bearing a number of conidia at the top which appear as white tufts; in addition to conidia 18-30 x 8-10 um, the anamorph produces thick-walled cells (chlamydospores) that are 60-90 x 15-20 um.

Hypomyces rosellus © Michael Beug

GLOSSARY

perithecium (plural perithecia) - a nearly spherical, ovoid, pear-shaped or beaked (flask-like) body bearing asci in the interior, with or without paraphyses, with an opening, characteristic of the pyrenomycetes such as Cordyceps, Xylaria, Claviceps, and Podostroma

subiculum - a net-like, wool-like, or crust-like growth of mycelium under fruiting bodies (in this case supporting the perithecia)

REFERENCES

1. Arora, David. 1986. Mushrooms Demystified. Ten Speed Press.
2. Gibson, Ian, Eli Gibson, Bryce Kendrick. 2008. MatchMaker: Mushrooms of the Pacific Northwest. CD program.
3. Pöldmaa, Kadri, Gary J. Samuels. 1999a. “Aphyllophoricolous species of Hypomyces with KOH-negative perithecia” Mycologia 91(1): 177-199.
4. Pöldmaa, Kadri, Ellen Larsson, & Urmas Köljalg. 1999b. “Phylogenetic relationships in Hypomyces and allied genera, with emphasis on species growing on wood-decaying homobasidiomycetes” Can J. Bot. 77: 1756-1768.
5. Rogerson, Clark T. 1971. “A new species of Hypomyces on Helvella” Mycologia 63: 416-421.
6. Rogerson, Clark T., Gary J. Samuels. 1989. “Boleticolous species of Hypomyces” Mycologia 81: 413-432.
7. Rogerson, Clark T., Gary J. Samuels. 1993. “Polyporicolous species of Hypomyces” Mycologia 85: 231-272.
8. Rogerson, Clark T., Gary J. Samuels. 1994. “Agaricolous species of Hypomyces” Mycologia 86: 839-866.

INDEX

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